Systematic Quantification of Neurotrophic Adipokines RBP4, PEDF, and Clusterin in Human Cerebrospinal Fluid and Serum

Abstract Context Data on the presence/quantification of neurotrophic adipokines retinol-binding protein-4 (RBP4), clusterin, and pigment epithelium-derived factor (PEDF) in human cerebrospinal fluid (CSF) are scarce migration these across blood-brain barrier (BBB) is uncertain. Objective This work aimed to quantify RBP4, PEDF, clusterin paired serum CSF samples patients undergoing neurological evaluation. Methods A total 268 (109 male, 159 female) were included. Adipokine concentrations measured by enzyme-linked immunosorbent assay duplicate. Results RBP4 was abundant (mean, 31.9 ± 24.2 μg/mL). The approximately 145 times higher than (CSF ratio, 8.2 4.3 × 10–3). PEDF detectable 30.2 11.7 μg/mL) 25 42.3 15.6 Clusterin with mean levels 346.0 114.6 μg/mL, which 40 29.6 23.4 correlated positively levels, increased overweight/obese type 2 diabetic patients. all 3 BBB dysfunction. inflammatory parameters. In detail, only showed kinetics associations that mandatory for a putative mediator fat-brain axis. Conclusion permeable increase measure represents an adipokine promising